Tag: Construction

Start a Rainbow Restoration Franchise, Rainbow Restoration Franchise Opportunity for Sale #rainbow #restoration, #rainbow #restoration #franchise, #service #franchises, #restoration #franchises, #construction # # #building #franchises, #commercial #cleaning #franchises, #carpet #cleaning #franchises, #b2b #franchises


Rainbow Restoration

Description: With over 30 years of experience, Rainbow International Restoration is one of the best-known and most respected names in the business. As an added bonus, Rainbow has built strong relationships with many of the largest insurance companies. These relationships help drive more business to our franchisees. Opportunities: Single, multi-unit opportunities available in the US. Business Type: Franchise. Minimum Cash Required: $35,000. Financing Assistance: Yes, through a third party. Training Provided: Yes.

15% off franchise fee

Special Veterans Offer
See Sidebar for More Details

A Rainbow International Restoration franchise is more than a business; it is a way to help people recover from loss by restoring value, stability and peace of mind. With services in mold removal, cleanup, water damage and fire and smoke damage, it s an industry less affected by the economy, and more driven by claims.

At Rainbow, our focus is on helping our franchisees meet and exceed their financial, professional and personal goals. Our expertise lies in providing the right tools, support and environment to turn those goals and dreams into a reality. We see it happen every day. Why shouldn t it happen to you?

(Jason Barck, Rainbow International Restoration franchisee, Des Moines, IA)

You invest in us. We invest in you.

With franchising, you get the independence of starting your own business with the security of joining forces with a nationally recognized brand. The combination of your energy and ambition with the experience and expertise of a larger company is explosive in its potential for success .

What you can expect:

  • Proprietary System. Our unique business model is designed for improving overall profitability and sustainability, including state of the art software to streamline operations.
  • Comprehensive ongoing training and support. Our Sure Start training helps prepare you for many aspects of operations including how to manage your finances. A dedicated Franchise Consultant will coach you for your first year. Franchisees in their second year of operation and after will be assigned a Regional Service Manager to advise them when needed.
  • Registered trademarks and a designated territory. We make sure you are protected.
  • Marketing, marketing, and more marketing. Superior marketing strategies and support offered through a 4-Pillar Marketing System:
      1. Web. Leading web presence, national and customizable website, bold online social media presence, SEO optimization, and reputation management
      2. Media. National and local media coverage and PR, consistent advertising
      3. Engagement. An online software system to receive client feedback and ensure their satisfaction, a referral program to increase a word-of-mouth advertising, automated personalized in-home service reminders and marketing pieces, back marketing email drip campaigns
      4. Continuous innovation. The Rainbow Restoration marketing team keeps ahead of trends and is always looking and learning new ways to keep the Rainbow Restoration brand the most recognized restoration brand in the business.
  • Nationwide networking support. You ll have over 320 fellow Rainbow International Restoration franchisees to consult with, PLUS the opportunity to meet up at Regional and National Conferences. That s a LOT of people to talk shop with.
  • Vendor Discounts. Cut your expenses with ProTradeNet, a program offering preferred discounts through membership that includes annual rebates.

(Rick Swango, Rainbow International Restoration franchisee, East Central Kansas)

And there s MORE

  • Recession proof business
  • Strong relationships with some of the largest insurance companies for referrals
  • Rainbow International Restoration was established in 1980 that s over thirty years of expertise in the business. We deliver well-established restoration procedures based on a results-driven business model designed to build sustainable profit.

Fill out the form below for more information on Rainbow Restoration!

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Accredited Online Construction Management Degree Programs #construction #management #degree #san #diego


Accredited Online Construction Management Degree Guide for 2015

As a construction manager, you will be involved in various administrative, business and managerial aspects of building projects. This can include selecting a crew of workers and architects, choosing building materials and making sure that your project stays within its budget and schedule. There are several different educational pathways including a certificate, a two-year associate degree, a four-year bachelor s degree and even a two-year master s degree. Although the specific curriculum for each type of program differs, common core courses cover topics like construction site safety and project management.

The construction manager is often the key leadership figure in a building project, both on the ground and in the office. They are typically responsible for project budgets, payrolls, construction methods, collaboration with engineers and architects, compliance with building codes and much more.

As a construction manager, you will have to select a team of architects, contractors and trade workers for your projects. You must also coordinate the activities of these different types of workers and ensure that progress on your projects stays on schedule. This often requires you to recognize and eliminate any factors that might slow the project time.


Check the Guide to Accreditation in Higher Education to learn more about accreditation and how to determine if the program you select meets the necessary standards.



Some of the most common classes that you can expect to have in an associate or bachelor s degree construction management program include:

  • Construction Project Management offers methods and techniques for preparing accurate cost estimates for building construction projects. You will learn practical strategies to help you manage and coordinate all the different types of specialists who are involved in construction projects.
  • Construction Safety deals with accident prevention, essential in the construction industry, so you will find this course on almost every construction management curriculum. You will learn how to identify common construction-site hazards, use standard building safety techniques and comply with building codes and regulatory requirements.
  • Construction Management Internships usually require you to work in the field at a building construction office. Internships usually require students to assist a professional construction manager with common tasks like scheduling, cost estimating, project planning and contract administration.

Certificate in Construction Management

These education programs are usually for students who already have some hands-on experience in construction management. In addition to certificate programs that are available through traditional construction management schools, professional associations like the Construction Management Association of America (CMAA) also offer certificate programs.

  • Credit hours/length of study: 9-18 months
  • Employment prospects: entry-level construction management positions

Associate Degree in Construction Management

You will find most construction management associate degree programs at two-year community colleges and technical colleges. Although there are many schools that still offer an associate in construction management, you should be aware that an increasing number of employers now view a bachelor s degree in construction management as the entry-level degree for this profession.

  • Credit hours/length of study: two years
  • Employment prospects: entry-level construction management positions

Bachelor s Degree in Construction Management

Schools that offer a four-year bachelor s degree in construction management train students for effective leadership on a construction site.

  • Credit hours/length of study: four years
  • Coursework: construction management, construction practices, legal liabilities and requirements of building projects

Master s Degree in Construction Management

Junior construction managers who already have several years of work experience are some of the most common students who pursue construction management training at the masters level.

  • Credit hours/length of study: two years
  • Employment prospects: senior or more advanced construction management jobs


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Online courses and career resources #online #courses, #distance #education, #resume #writing, #cover #letters, #career #resources, #construction #project #management #courses


Construction project management courses

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Looking for Project Management opportunities in Australia ?

There are currently 32,663 Project Management job openings in Australia*. The average salary for a Project Management job in Australia is $82,056.

The Australian Government Department of Employment estimates a projected employment growth to 2018 of 88,700 jobs for the Project Management industry in Australia .

Gaining a Project Management qualification will significantly increase your career prospects for Project Management jobs in Australia. Studying an online course with an accredited Australian provider gives you the flexibility you need to study at your own pace.

Employment in the Project Management industry in Australia

Australia has a population of 23,625,031, with a total of 11,645,500 people currently employed. The average job seeker age for Australia is 37 years and the current unemployment rate sits at 5.7%. Part-time workers make up 46% of the workforce in Australia .

Education in Australia

  • The percentage of people employed in Australia with a bachelor’s degree or higher qualification: 29%
  • The percentage of people employed in Australia with a cert III or higher VET qualification: 31%
  • The percentage of people employed in Australia without a post-school qualification: 36%

Disclaimer: Career FAQs Pty Ltd ABN 39 299 617 067 (Career FAQs) markets the education and training services of a range of Australian tertiary course providers, and receives a commission from them for each prospective student. Career FAQs is not an education provider. All material and information regarding our education providers and their courses – on our site, via email, or over the phone – is delivered through our capacity as their agent. The material shared with you on the website is provided as general information only. It is not intended as professional advice, and should not be taken as such. All information is provided in good faith, and is believed to be accurate and current as at the date of publication. However, Career FAQs provides no guarantee that any information or material on the website, or linked websites, will be accurate or complete. Please see our privacy policy for more information.

Career FAQs 2017

Career FAQs markets a range of courses from leading Australian tertiary education providers and receives a commission from them for each prospective student.

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Ivey Mechanical-What We Do #ivey #mechanical, #mechanical #contractor, #mechanical #construction


Ivey Mechanical Acquires Optimech

Ivey Mechanical Company, LLC, headquartered in Kosciusko, MS is pleased to announce the acquisition of OptiMech, LLC. OptiMech is a cutting edge provider of design-build mechanical solutions serving the needs of the industrial and institutional marketplace. The firm offers a wide portfolio of integrated engineering, mechanical laboratory and process construction solutions. OptiMech is located in Hendersonville, TN and focuses primarily on the data center, laboratory, industrial and food and beverage market segments across the country.

Denny Terrell, President of Ivey Mechanical Company states, “This acquisition will strengthen the operations of both groups by sharing resources, knowledge and experience with very little overlap. The people in any organization are what make the difference, and we are extremely excited about the talent and professionalism that exists within the OptiMech group. For Ivey, this acquisition will add approximately 15% in additional revenues and in the number of employees. Also, it will substantially increase the workload in our fabrication shops located in Kosciusko”.

What We Do

Ivey Mechanical Company is a nationally recognized mechanical contractor specializing in projects that demand the most exacting mechanical expertise. Ivey is a merit-shop contractor. Ivey has hundreds of management and field professionals with experience in all types of mechanical construction. With these industry veterans, Ivey has successfully completed projects in more than 30 states. Each Ivey business unit is staffed with project management professionals who have complete responsibility for specific Ivey projects. As a full-service mechanical contracting firm, Ivey Mechanical Company is organized around its construction, fabrication, and service groups with an annual volume in excess of $200 million.

Preventative Maintenance

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Outer Banks – Albemarle Termite – Pest Control – Elizabeth City, NC – Pest Control Services #new #construction, #residential, #fast #response # # #great #customer #service #is #our #guarantee!, #specializing #in:, #termite #control, #conventional #treatments, #environmentally #safe #termite #baiting #systems, #moisture #control,, #dehumidification #systems, #we #offer #affordable, #pest #control #programs, #spiders, #roaches, #rodents, #fleas, #silverfish, #earwigs #and #more…, #pre-treatments #for #new #homes, #new #wood #treatment #technology, #conventional, #baiting, #insect #reports #for #home #sales #or #refinance, #commercial #division, #hospitals, #schools, #residential # # #commercial, #insured, #online #access:, #pay #your #bill, #bed #bug #control, #pest #control #quotes #over #the #phone, #certificates, #pest #control #services, #moisture # # #vapor #control, #moisture #control, #pest # # #termite #control


Albemarle Termite Pest Control

Ask About Integrated Pest Management, Beg Bug Control, History & More, Locally Owned & Operated, of Experience Serving the Albemarle Area, Professional Technicians With Years, Proactive Protection, Residential & Commercial, Retail, Schedule Appointme.

Albemarle Termite & Pest Control – Outer Banks in Elizabeth City, NC – Pasquotank County is a business with Technicians on staff and specialized in Ants, Restaurants, Government, Cockroaches, Earwigs, Termites, Industrial, New Homes, Residence and Vermin. Albemarle Termite & Pest Control is listed in the categories Pest Control Services, Moisture & Vapor Control, Moisture Control and Pest & Termite Control and offers New Construction, Residential, FAST RESPONSE & GREAT CUSTOMER SERVICE IS OUR GUARANTEE. SPECIALIZING IN. Termite Control, CONVENTIONAL TREATMENTS, Environmentally Safe Termite Baiting Systems etc. If you did business with Albemarle Termite & Pest Control, please leave a review and help us improve and help other people. Also, don’t forget to mention Hubbiz.

Pest Control Services, Moisture Vapor Control, Moisture Control, Pest Termite Control


Ants, Restaurants, Government, Cockroaches, Earwigs, Termites, Industrial, New Homes, Residence, Vermin

Guarantees, Environmentally Aware, Environmentally Conscious, Environmentally Friendlly, Environmentally Responsible, No Environmental Harm


New Construction, Residential



Professionals on Staff:

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Office Remodel Dallas TX #office #remodel #dallas #tx,dallas #remodeling,commercial #remodeling #dallas,commercial #contractor #dallas,commercial #painting #dallas,commercial #general #contractor,contractor #dallas,commercial,tenant #construction,tenant #improvement,commercial #contractor,office #remodel,building #remodel,commercial #remodel,remodeling,remodel,construction,renovation,renovations,office #remodeling,office #renovation,office #layout,remodeling,consruction,steel #construction,commercial #remodeling,plano,frisco,dallas,mckinney,fort #worth,ft.worth,arlington,richardson,coppell


Honesty, integrity, and Quality Workmanship

G.C. Construction, Inc. is a Dallas / Ft. Worth Texas based commercial general contractor that has been building extraordinary projects since 1986. Our quality workmanship has earned us a reputation as one of the area’s premier contractors. We specialize in office remodel / commercial interior finish out, shell construction and design build. We offer commercial remodeling in Dallas and the surrounding areas which we provide with a quality foundation of unwavering ethics.

Distinctive Office Space

We will build you the office you want, period. No surprises or disappointments. If you can dream it, we can build it. We maintain control throughout the design and construction process to ensure that your office is built to the highest quality standards.

We can be contacted at the phone number listed above or you can complete our quick contact form and we will contact you as soon as possible.

Office Remodel Dallas TX

We manage all stages of your project. From the pre-design phase of a project through construction and delivery. We form alliances with property owners, managers, architects, leasing agents, office tenants and specialized trades people.

G.C. Construction, Inc. Guarantees:

  • On schedule completion
  • Cost-effective process from beginning to end
  • Final product exceeds expectations
  • Honesty, integrity, and great value

We are known for on time completion with an excellent finished product


Gene and his crew are basically wrapping things up on the construction/expansion, i wanted to say how pleased i am with the new space.
Read more.

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Service Management #serviceability, #utility, #maintenance, #usefulness, #business, #office, #construction #management, #service #desk, #help #desk, #safety, #enterprise #software, #service #solution, #application, #processes, #workflow, #scalable, #cmdb, #discovery, #event #monitoring, #mobile, #adaptable


  • One Solution
    Multi-faceted. Multi-tenant.
    you decide ?

    Workflow driven notifications allow the two-way communication from office to staff to edit and resolve data entry issues. Issues such as expense reimbursement require attached receipts. The user can easily take a snapshot photo of a receipt, attach to a request and satisfy a receipt request.

    As the timesheet. expenses and mileage request proceeds through approval processes, the user sees changes to their request in real-time. Users may login at any time to review status changes and follow processing to completion.

    Service and Support personnel need application access. The tablet interface provides a convenient secure connection to verify support ticket information, check parts availability and a host of other assignable app permissions. Mobility support includes two-way customer notifications and responses.

    Always Up-to-date

    We provide sand-box access to allow for pre-release updates to our application. This provides a functional interface designed to demonstrate the anticipated changes and offers our users a genuine ability to participate in application change design and adoption.

    Communicate directly with your customer via built-in chat functionality. Provide technical assistance and references to your solution knowledgebase for self-help information sharing.


    While document management is not new, it is vitally important to today’s successful businesses. Create, attach . and store any reference document as required by business processes. Need to create a new document that must have a signature? Our on-the-fly document creation process includes signature fields that are functional in a touch screen tablet environment.

    Safety management designed for the construction industry and safety programs in general, is also part of the solution.

    e -Vis asset visualization (included) provides for easy-to-see visual mapping of and selected asset(s) and interconnected relationships.

    Think it over, schedule a demonstration and kick the tires or use our contact page to reach out to us.

    Safety Compliance

    Resources you require to manage a Safety Compliance program.

    We help plan . process and manage your Health & Safety Compliance program. We tie all the diverse information flowing in from projects into a coordinated compliance effort. Monitor and track issuance of Safety-Designated Inventory .
    Integrated barcode recognition provides for easy asset control of safety equipment as items are issued (and recovered ) to staff personnel.

    Near Miss Events

    A Near Miss is an unplanned event that did not result in injury, illness, or damage � but had the potential to do so.

    Key Points

    • Incidents occur every day at the workplace that could result in a serious injury or damage.
    • A near-miss program may help prevent future incidents.
    • One problem that companies must overcome is the employee fear of being blamed after reporting a near miss.

    Let us look at the accident pyramid adapted from Heinrich in Figure 1.

    The pyramid estimates that: for every fatal workplace accident, 3000 incidents did not cause any injuries. In other words, you are presented with 3000 opportunities to stop that fatality from taking place. Companies are not expected to and may not be able to ideally capture all the 3000 near miss events that occurred, given the volume of reports to manage. Just turning in and acting on 1% of that volume, would present companies with 30 opportunities to take corrective or preventive action.

    Resource Management

    We define our construction document management system as the integral control and organization of all documents within our Construction Management Solution. Monitor and track site inspection survey documents, work change orders and any type of important document that you wish to link to a site record.

    Staff Training

    Let our Training Application – coordinate both internal and external staff training. Schedule and monitor ToolTalks. by job site and/or by attendee. Manage attendance and performance of staff training. Training calendar offers a visual queue to help evaluate and coordinate education processes,

    Certificate Management

    Are your Staff Certified

    Staff certification is more then an award for completing a training program. In many instances an employee’s job performance and employ-ability rest on holding and maintaining a level of certification unique to a designated job skills. Compliance and validation of certification rests with the employer.

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  • Quality In Construction #construction #quality #management #plan


    Online Collaboration and Document Management Cloud solutions for construction industry are everywhere these days. Here are three lists of Apps and Cloud tools for Document Control and Inspections that are on the market at the moment: 7 Cloud-based collaborative solutions for construction industry 9 Continue Reading

    RFIs are one of the most important and useful processes in today’s construction projects and it is an extremely important tool for every size and Continue Reading

    If you are a Quality Manager who has worked in at least 3 construction projects you would have probably started recognizing a pattern: other Continue Reading

    Online Collaboration and Document Management Cloud solutions for construction industry are everywhere these days. Here are three lists of Apps and Cloud tools for Document Control and Inspections that are on the Continue Reading

    Normally, we don’t really talk about Health Safety stuff at QualityInConstruction.com but we recently discovered an online tool that seems really promising. The tool is called HandsHQ and it is introducing Continue Reading

    *For All the latest News, Reviews and Top Notch tips visit the new website. Apps For Construction Apps and SaaS tools for construction industry will soon become a very prominent part of an Engineer’s everyday Continue Reading

    *For All the latest News, Reviews and Top Notch tips visit the new website. Apps For Construction It seems like ages ago when people first suggested the use of tablets at construction sites, but today it feels Continue Reading

    On the 2nd of January 2015, a 80-metre wind turbine has collapsed in Northern Ireland. The turbine was one of 8 on the Screggagh wind farm on Murley mountain near Fintona in County Tyrone of Northern Continue Reading

    A major quality failure has recently been recorded last Tuesday in London, UK. An area around the 47-storey Leadenhall Building in the city of London has been cordoned off because a part of a bolt from the frame Continue Reading

    It’s been 13 years since the Collapse of the WTC buildings on the 9/11/2001. We will not re-produce the thousands of conspiracy theories about the event (a Google search turns up about 16.000.000 results..) but Continue Reading

    Everybody who works in construction knows that things can go wrong. Everybody knows that even the slightest mistake or lapse somewhere can lead to a disaster. Nonconformities in construction industry don’t only lead Continue Reading

    These are the 5 worst Quality Failures in Construction and Building industry: 5. Hyatt Regency Skywalk Collapse, Kansas City. USA 1981 4. Deepwater Rig Oil Spill, Gulf of Mexico, 2010 3. Sampoong Department Continue Reading

    A 15ft-deep hole appeared on M2 motorway in Kent, UK on 11th of February 2014. The 15ft chasm that has closed the motorway, also caused traffic chaos across the area in UK and it was backfilled within a week Continue Reading

    In order for a construction company or a construction project to function properly, internal processes and procedures that will set up the frame of its basic operational activities are almost always required Continue Reading

    CAR is a well known abbreviation to everyone who works in a Quality- related role across all industries (and not only in construction). For those who are not familiar with the term, CAR stands for Corrective Continue Reading

    Document Control in a construction site is a very critical process and not settng it up properly from day 0 of the project, could have significant implications to the final product ( or project ). It’s also a Continue Reading


    NCR and CAR Templates

    Over 8000 construction professionals have already subscribed!

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    Rowells Sewer – Drain, NH Plumbing, Line Cleaning, Pipe Repair, Portable Toilets #bedford, #manchester, #merrimack, #lakes #region, #laconia, #tilton, #center #harbor, #meredith, #moultonborough, #plymouth, #gilford, #sanbornton, #bristol, #holderness, #ashland, #franklin, #belmont, #septic, #rowells, #septic #cleaning, #septic #tank,sewer #pipes, #tank #location, #tank #pumping, #thawing #frozen #lines, #septic #video #inspection, #water #jetting, #septic #inspections, #septic #installations, #drain #line #maintenance, #commercial #pump #station, #local #drain #jetting, #septic #tanks #and #systems, #blocked #sewer #lines, #new #construction #septic #installation, #buying #home #inspection, #clogged #drain #lines, #pump #chamber #maintenance, #septic #design, #locating #septic #tank, #real-estate #inspections






    More than septic tank pumping; Rowell’s Services is New Hampshire’s leading resource for plumbing, heating, air conditioning, electrical, septic and drain clearing services.


    We are happy to provide your preventive maintenance plans for your heating and air conditioning systems.

    Family owned and operated since 1983.

    We are proud to announce the addition of Smoke Testing to our professional line of services. A cost effective and safe way to determine the location of unwanted plumbing odors.

    We offer professional, immediate service for both residential and commercial customers. Ask about our Automatic Maintenance Programs so you don’t have to worry about scheduling!

    Don’t forget to have your septic tank pumped on a regular basis.

    Schedule your appointment today the

    Sep 6 – Pumper | Cleaner Magazine 2016

    We know you likely don’t subscribe to Pumper Magazine, or Cleaner Magazine. So, we wanted to share with you some great articles about Rowell’s Services from 2016! We would be honored for you to take a few minutes to preview these exciting articles!
    Pumper Magazine, August 2016 Dad Would Be Proud of His Family’s Pumping Success: Rowell’s Sewer Septic uses effective marketing, a company jingle and new headquarters to exceed all expectations. Cleaner Magazine, March 2016 Building a Family Drain Cleaning Business: Cleaning and pumping provide a solid foundation for family business’ growth and diversification

    Senior Citizen
    Veteran Discounts!

    5.00 Off

    Proud Member of the NH Association of Septic Haulers (NHASH) and the Granite State Designers and Installers Association (GSDI)

    Tilton, NH 03276

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    Single Domain Antibody Library – Creative Biolabs #single #domain #antibody #library #construction #and #screening, #domain #antibody #sdabs #vhh #vnar, #immunized #sdab #library, #synthetic #sdab #library, #pre-made #human #sdab #library


    Phage Display Platform

    Creative Biolabs develops and commercializes a full range of integrated innovative services that are based on phage display technology. We have.

    Protein Engineering Platform

    Creative Biolabs can offer advanced protein engineering platform for your specific project, including high-scale expression, crystallization and characterization.

    Membrane Protein Platform

    Creative Biolabs has established custom membrane protein and membrane protein antibody production platforms for antibody discovery.

    Hybridoma Platform

    Creative Biolabs provides a full range of services based on our matured hybridoma platform. Our featured services involve the custom monoclonal antibody production, from.

    Single Domain Antibody Library

    Creative Biolabs is a well-recognized leader in single domain antibody (sdAb) library construction and screening services. Our unique services including:

    Why single domain antibodies are preferred?

    Single domain antibodies represent the smallest antibody that was proven of diagnostic and therapeutic usefulness. They are antibody fragments that engineered from single monomeric variable domains of either camelids’ heavy-chain antibody (VH H) or cartilaginous fishes’ IgNAR (VNAR ). On the other hand, it is also possible to develop single domain antibodies from camelized human antibodies.

    In particular, single domain antibodies combine the benefits of conventional antibodies with important features of small molecule drugs. They are able to bind to hidden epitopes that are not accessible to whole antibodies. In addition, in comparison with small molecule drugs that target enzyme active sites and receptor clefts, single domain antibodies have the potential of greater affinity and selectivity, thus promising lower side effect and better efficacy. Furthermore, because of their small size (they are only 1/10 the size of a whole antibody), single domain antibodies can penetrate tissues faster than other antibodies and even break through the blood-brain barrier (BBB), which turns them into excellent candidates for central nervous system disease therapies. Single domain antibodies have higher stability during the changes of temperature and chemical environments and great potential for gastrointestinal stability and oral availability. Also, thanks to its simple nature of a single-chain peptide, single domain antibodies have exceptional drug format flexibility and allow efficient drug discovery and development.

    Construction of Immunized Single Domain Antibody Library

    In terms of the advanced phage display technology, Creative Biolabs has unparalleled capabilities for the construction of VH H or VNAR based single domain antibody libraries through immunized camel, llama, alpaca or shark. In comparison with naïve libraries, immune libraries usually produce antibodies of greater affinity, thus avoiding time-consuming in vitro antibody affinity maturation effort. Antibodies from naïve libraries frequently require affinity maturation before they are useful due to affinity issues. Furthermore, in comparison with naïve libraries, which usually require a size of 1-10 billion independent clones to be useful, an immune library can be much smaller. Our experience showed that immune libraries with a size of 1-10 million variants could be sufficient to produce excellent antibodies.

    Our scientists have extensive experience in cloning the single domain antibody repertoire from immunized plasma cells into our phage display vectors. By reverse transcription and polymerase chain reaction, a library of single domain antibodies containing 10-100 million clones is regularly produced.

    Construction of Synthetic Single Domain Antibody Library

    Creative Biolabs also has sufficient expertise in constructing synthetic naïve single domain antibody libraries. By nature, single domain antibodies developed from either VH H or VNAR are more stable and soluble, although their immunogenicity can be higher than single domain antibodies engineered from human antibodies. If the immunogenicity of the single domain antibodies is not a concern, a synthetic VH H or VNAR single domain antibody library can be made by randomizing the CDR1 and CDR3 using trimer codon technology.

    In case the immunogenicity of single domain antibodies is a concern, our services allow the construction of camelized human single domain antibody libraries which are based on the monomerization of human antibody heavy chain framework. The binding region of normal human IgG consists of two domains (VH and VL) which tend to dimerize or aggregate because of their lipophilicity; the monomerization will be accomplished by replacing lipophilic with hydrophilic amino acids at a few proprietary positions. Such synthetic libraries represent a good source of single domain antibodies against self, non-immunogenic, and toxic antigens since the libraries are usually sufficiently vast and diverse.

    Screening of Single Domain Antibody Library

    To select interested single domain antibodies with high affinity and specificity, scientists from Creative Biolabs can offer high-quality screening service for phage-displayed sdAb libraries. In particular, we are specialized in isolating single domain antibody inhibitors that can access cavities within molecular targets such as enzyme active sites and receptor clefts. Meanwhile, a method based on heat denaturation of single domain antibodies on the surface of phage has also been developed to select non-aggregating single domain antibodies.

    Single Domain Antibody Production

    Creative Biolabs has established the novel sdAb production platforms that enable the generation of target-specific single domain antibodies within 8 weeks. We have produced a large number of single domain antibodies with high affinity and specificity against a large number of distinct targets, including cytokines, cell surface receptors, tumor cell markers, viral antigens, and enzymes. Of note, single domain antibodies are perfectly stable polypeptides harboring the full antigen-binding capacity of conventional antibodies. This unique structural and functional property render these molecules ideal candidates for new generation of antibody therapeutics.

    To meet the special needs of our clients on single domain antibody, Creative Biolabs has combined our cutting-edge techniques with advanced phage display technology to offer a series of services including sdAb library construction, screening, and sdAb production. Our scientists are confident in tailoring the most appropriate protocol to develop high-quality single domain antibodies for our clients’ research purpose.

    Nuttall, S. D. (2012) ‘Overview and discovery of IgNARs and generation of VNARs’, Single Domain Antibodies: Methods and Protocols. 27-36.

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